The development of RNA sequencing technology has been driven by two fundamental objectives: the ability to accurately analyze the entire transcriptome and the capacity to dynamically capture RNA modification information. Conventional short-read-long sequencing technologies encounter limitations due to fragmented splicing, impeding the accurate identification of full-length transcripts... https://www.cd-genomics.com/epigenetics/resource-ont-direct-rna-sequencing-principles-workflow-analysis.html